Equipment

Transmission electron microscopes

TEM

The facility has a three transmission electron microscopes available for imaging samples at ambient and cryo temperatures.

FEI Tecnai Spirit 120 kV

FEI F20 cryo

Jeol 2200 FS

Scanning electron microscopes

fly eye

The facility has a number of scanning electron microscopes available for imaging biological samples. This also includes a focussed ion beam scanning electron microscope.

Zeiss Merlin resolution SEM

Zeiss NVision FIBSEM

Zeiss Crossbeam 560 FIBSEM

 

Block face scanning microscope

ultramicrotome

The most recent addition to the facility is the Gatan 3view microscope. The microscope uses a combination of scanning electron micrscopy and an internal ultramicrtome to produce a series of images. With each section nm of resin are removed using the ultramicrotome and the new surface is then imaged. This allows the user to move through the sample and produce a detailed stack of information from which measurments can be taken and models can be made.

 

Focussed ion beam microscopes

The facility has two focused ion beam micrscopes avaliable for imaging biological samples. The microscope uses the ion beam to mil away the surface of the block revealing a new surface and producing a series of images that move through the biological sample. 

Zeiss Nvision FIBSEM 

Zeiss Crossbeam 560 FIBSEM 

Sectioning equipment

serial sectioning

The facility has a number of ultramicrotomes for both ambient and cryo temperature cutting. This includes two Leica UC7 machines, two Leica UCTs, and an RMC. Some of these ultramicrotomes are equipped with video facilities.

 

Cryo preparation equipment

ultramicrotome

The facility has a number of ultramicrotomes for both ambient and cryo temperature cutting. This includes two Leica UC7 machines, each of which is fully equipped with video facilities.

 

Plunge-freezing apparatus

serial sectioning

Vitrobot Marc IV.  Device for automated vitrification of acqueous suspension for cryo TEM imaging. The interface with touch screen is easy to use and parameter as humidity, temperature, force blot, number of blot can be programmed for each individual application. It ensure high quality and reproductible freezing of samples.